REACCIÓN DE LA POLIMERASA EN CADENA: PCR SOBRE PAPEL.

A partir de una molécula de ADN parenteral y los cebadores correspondientes se van a obtener una serie de cadenas nuevas, a partir de sucesivos ciclos.

El objetivo de esta práctica es visualizar como este sistema puede amplificar una secuencia determinada y elegida por nosotros (que va a depender de los cebadores que introduzcamos).

MOLÉCULAS DE PARTIDA

Molécula de ADN:

3'-ATGCTGGGCCACAGTTTCAATCGAATCAGT-5'

5'-TACGACCCGGTGTCAAAGTTAGCTTAGTCA-3'

Cebadores:

5'-CCCGG-3'

3'-TCGAA-5'

RESULTADOS

El número y el tipo de cadenas de ADN que hemos obtenido, ha sido:

Cadena	Número
3'-ATGCTGGGCCACAGTTTCAATCGAATCAGT-5' 5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'	1
5'-TACGACCCGGTGTCAAAGTTAGCTTAGTCA-3' 3'-ATGCTGGGCCACAGTTTCAATCGAA-5'	1
5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3' 3'-GGGCCACAGTTTCAATCGAA-5'	4
3'-ATGCTGGGCCACAGTTTCAATCGAA-5' 5'-CCCGGTGTCAAAGTTAGCTT-3'	4
3'-GGGCCACAGTTTCAATCGAA-5' 5'-CCCGGTGTCAAAGTTAGCTT-3'	22

Los pasos que se han seguido para completar los ciclos, se muestran a continuación.

1º CICLO

3'-ATGCTGGGCCACAGTTTCAATCGAATCAGT-5'

5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'

5'-TACGACCCGGTGTCAAAGTTAGCTTAGTCA-3'

3'-ATGCTGGGCCACAGTTTCAATCGAA-5'

2º CICLO

3'-ATGCTGGGCCACAGTTTCAATCGAATCAGT-5'

5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'

5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-TACGACCCGGTGTCAAAGTTAGCTTAGTCA-3'

3'-ATGCTGGGCCACAGTTTCAATCGAA-5'

3'-ATGCTGGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3º CICLO

3'-ATGCTGGGCCACAGTTTCAATCGAATCAGT-5'

5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'

5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'

3'-GGGCCACAGTTTCAATCGAA-5'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-TACGACCCGGTGTCAAAGTTAGCTTAGTCA-3'

3'-ATGCTGGGCCACAGTTTCAATCGAA-5'

3'-ATGCTGGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-ATGCTGGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

4º CICLO

3'-ATGCTGGGCCACAGTTTCAATCGAATCAGT-5'

5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'

5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'

3'-GGGCCACAGTTTCAATCGAA-5'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'

3'-GGGCCACAGTTTCAATCGAA-5'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-TACGACCCGGTGTCAAAGTTAGCTTAGTCA-3'

3'-ATGCTGGGCCACAGTTTCAATCGAA-5'

3'-ATGCTGGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-ATGCTGGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

3'-ATGCTGGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5º CICLO

3'-ATGCTGGGCCACAGTTTCAATCGAATCAGT-5'

5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'

5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'

3'-GGGCCACAGTTTCAATCGAA-5'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'

3'-GGGCCACAGTTTCAATCGAA-5'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTTAGTCA-3'

3'-GGGCCACAGTTTCAATCGAA-5'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-TACGACCCGGTGTCAAAGTTAGCTTAGTCA-3'

3'-ATGCTGGGCCACAGTTTCAATCGAA-5'

3'-ATGCTGGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-ATGCTGGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

3'-ATGCTGGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-ATGCTGGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'

5'-CCCGGTGTCAAAGTTAGCTT-3'

5'-CCCGGTGTCAAAGTTAGCTT-3'

3'-GGGCCACAGTTTCAATCGAA-5'